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Description
This high fidelity DNA Polymerase is a thermostable enzyme engineered from Pyrococcus furiosus, by fusing it with the small, non‑specific DNA‑binding protein Sso7d from Sulfolobus solfataricus. This fusion significantly enhances the enzyme’s processivity and performance while preserving its 5′→3′ polymerase activity and intrinsic 3′→5′ proofreading exonuclease activity. As a result, Pfu‑sso7d generates highly accurate PCR products with substantially reduced error rate (>30x less errors than Taq DNA polymerase), making it an ideal choice for cloning, site‑directed mutagenesis, and other applications that demand low-error DNA amplification, even from complex or GC‑rich templates.
This product includes all reagents needed to perform a PCR reaction with the exception of dNTPs, primers and DNA template. It includes:
| Component |
Composition |
| Pfu-sso7d DNA Polymerase | 1 units/ul |
| 5x Pfu polymerase buffer | 50 mM Tris-HCl, pH 8.5, 250 mM KCl, 7.5 mM MgCl2, 50 mM (NH4)2SO4, 0.5% Triton X-100 |
Technical Information
| Product Manual | Click here |
| Certificate of Analysis | Click here |
| Key References | Menacho-Melgar, R., Yang T, and Lynch, MD., Instant Taq: Rapid Autoinducible Expression and Chromatography-free Purification of Taq polymerase. biorxiv., 25 September 2021. |