E. coli  "Instant Taq" + mCherry Positive control plasmid

E. coli "Instant Taq" + mCherry Positive control plasmid

RSA-002
Regular price
$149.00 USD
Sale price
$149.00 USD
Regular price
Sold out
Unit price
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Academic or non-profit institutions pay a one-time fee per principal investigator (PI). Commercial customers pay an annual fee. A Material Transfer and License Agreements are required.

-Orders placed after 15:00 ET are shipped the next business day.
-We take Purchase Orders (POs). Add to cart and select PO as the method of payment to receive more instructions, or contact us at support@rokebio.com.

Product Description

This is a genetically modified E. coli strain engineered to enable the easy expression and purification of Taq DNA Polymerase. Our approach combines protein autoinduction, equipment-free lysis and chromatography-free purification to yield high-quality DNA Taq Polymerase, equivalent to commercially available enzymes.

Eco-friendly: Whenever you buy Taq DNA Polymerase, waste and greenhouse gas emissions are generated to package and ship Taq to you. Reduce your carbon footprint by making your own reagents.

Minimal effort: Our method takes less than one hour of hands-on time regardless of making Taq DNA Polymerase from 1 or 10 shake-flasks. Since a single 20 ml shake-flask culture yields 5000 units of Taq DNA Polymerase (4,000 reactions), it takes less than one hour of your time to make a year’s worth supply of Taq.  

Accessible: Is your laboratory group not an expert or equipped for protein purification? It does not matter. This method uses simple steps and does not require ANY sophisticated protein purification equipment or expensive protein purification resins. You only need an incubator shaker, freezer and centrifuge for the whole procedure.

Eco-friendly: Whenever you buy Taq DNA Polymerase, waste and greenhouse gas emissions are generated to package and ship Taq to you. Reduce your carbon footprint by making your own reagents.

Minimal effort: Our method takes less than one hour of hands-on time regardless of making Taq DNA Polymerase from 1 or 10 shake-flasks. Since a single 20 ml shake-flask culture yields 5000 units of Taq DNA Polymerase (4,000 reactions), it takes less than one hour of your time to make a year’s worth supply of Taq.

Accessible: Is your laboratory group not an expert or equipped for protein purification? It does not matter. This method uses simple steps and does not require ANY sophisticated protein purification equipment or expensive protein purification resins. You only need an incubator shaker, freezer and centrifuge for the whole procedure.

Price Comparison

For a one-time fee, the strain is yours to use within your laboratory. As shown below, just purchasing the strain and making Taq DNA Polymerase from a single 20 ml shake-flask is cheaper than purchasing purified Taq. Making Taq from multiple shake-flasks is equally easy and dramatically decreases the cost of this reagent. Look under Product Description for more information

 Technical Information
Host Genotype  F-, λ-, Δ(araD-araB)567, lacZ4787(del)(::rrnB-3), rph-1, Δ(rhaD-rhaB)568, hsdR51, ΔackA-pta, ΔpoxB, ΔpflB, ΔldhA, ΔadhEΔiclR, ΔarcA, ΔompT::yibDp-ƛR-nucA( H184R)-apmR)
Plasmid

pHC-Kan-yibDp-Taq: high copy plasmid for the low phosphate auto-induction of Taq Polymerase, confers kanamycin resistance. 

(Control only) pHC-Kan-yibDp-mcherry: high copy plasmid for the low phosphate auto-induction of mcherry, confers kanamycin resistance. For the control plasmid map, click here

Antibiotic Resistance Kanamycin, Apramycin and Gentamicin
Product Manual Click here
Key References Menacho-Melgar, R., Yang T, and Lynch, MD., Instant Taq: Rapid Autoinducible Expression and Chromatography-free Purification of Taq polymerase. biorxiv., 25 September 2021.